INDEX:

Alkaline Phosphatase
Apoperoxidase
Chymotrypsin-USP
Cytochrome C
Deoxyribonuclease
Diaphorase
Galactosidase
Glucose Oxidase
Hyaluronidase

Peroxidase
Ribonuclease
t-Plasminogen Activator Inhibitor
(tPAI, Erythrina)
rSteptokinase
Trypsin-USP
Trypsin Inhibitor

(Soybean)
Urease

PRODUCT:
CYTOCHROME C

CAT. NO.:
  C10-1

SOURCE:
  Horse heart

FORM:
  A salt-free, freeze-dried powder predominantly in the oxidized forml

UNIT DEFINITION:
  The methods of assay used in our laboratories are as follows:
1) Percentage iron is determined spectrophotometrically with alpha-dipyridyl on a moisture-free basis.
2) Cytochrome C is fully reduced with Sodium Dithionite at pH 7.0 and absorbance measured spectrophotometrically at 550 nm.
3) The auto-oxidizability is determined by the carbon monoxide method of Tsou, C.L. Biochem J., 1951: 49, 362.
4) Purity is determined spectrophotometrically.

ACTIVITY:
  Reduced > 90%
Oxidized > 89.4%
Iron > 0.38%

STORAGE:
  Best stored at –20°C

ADDITIONAL
DATA:

  A550nm reduced/A278nm oxidized = >1.0
A550nm reduced/A550nm oxidized = >3.0

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